Influence of peracetic acid-ethanol sterilization on the biological and mechanical properties of the ACL graft


Sterilization of allogenic soft-tissue grafts, such as the anterior cruciate ligament (ACL), has become a legal requirement in Germany. Current sterilization techniques possess several disadvantages, so that alternative techniques are needed that do not interfere with the biological and mechanical properties of such treated tissue. The peracetic acid-ethanol (PAA) technique is an established method for bone-sterilization that has been employed successfully for a long time. In pre-studies we found no negative effect of PAA on the mechanical properties of soft-tissue grafts in-vitro. However, there are no studies on the efficiency of PAA for soft-tissue sterilization and no in-vivo studies exist. Therefore, we analyze the penetration capacity and disease prevention potential against virus, bacteriae and fungi in-vitro and examine the impact of PAA sterilization on the biological healing and mechanical properties of an ACL replacement graft in-vivo in a sheep model. Different stages, early (6 weeks), mid (6 months) and long-term (1 year) healing will be analyzed. Goal of this study is the possible introduction of a new, inexpensive sterilization technique of soft-tissue, which could not only be used for ACL reconstruction, but also for various areas of allograft transplantation.
Head of Project:

Dr.med.vet. Tanja Schmidt
Dr. Sven Scheffler
Charité - Universitätsmedizin Berlin
Department of Trauma and Reconstructive Surgery incl. Department of Orthopedic Surgery CCM/CVK
Additional Head of Project:

PD Dr. Weiler
Begin/End of Project:

01/2005 - 12/2012
Funded by:

Stiftung
Online-Information:

www.cmsc-online.de
Publications:

# Scheffler S.U., Scherler J., Pruss A., von Versen R. & Weiler A.: Biomechanical comparison of human bone-patellar tendon-bone grafts after sterilization with peracetic acid ethanol. Cell and Tissue Banking: 6(2) 109-15, 2005