Synthese des Na-Kanal-Proteins EnaC


The epithelial sodium-channel ENaC is considered to be rate limiting in blood pressure and hormone (aldosterone, Ang II, ADH) dependent fine-tuning of Na-resorption in the renal collecting duct. ENaC is discussed as an important pathophysiological factor for development of a salt-dependent high blood pressure. ENaC expression is hitherto analysed mainly at the level of transcription and assembling of the subunits ( ) in the cell membrane. mRNA and protein quantification data have shown, that during induction (salt-diets and hormone application) mRNA dependent posttranscriptional control plays an important role. The underlying molecular mechanisms, however, are not known. mRNA specific regulation of protein synthesis is mediated predominantly by interaction of RNA-binding proteins with untranslated regions of mRNAs (5'/3'UTRs). The project aims to analyse the role of the untranslated regions of ENaC mRNAs with respect to haemodynamic and hormonal control of ENaC expression. We plan to investigate nature and mode of action of regulative ENaC mRNA binding proteins and want to analyse their functional impact in cell culture and animal models.
Head of Project:

Prof. Dr. Bernd-Joachim Thiele
Charité - Universitätsmedizin Berlin
Institute of Vegetative Physiology CCM
Tel. +49 30 450 528184
Fax +49 30 450 528972
bernd.thiele@charite.de
Begin/End of Project:

01/2006 - 12/2008
Funded by:

Deutsche Forschungsgemeinschaft e.V.