Proteomische Charakterisierung der PDZ Domänen Spezifität

Aim of this project is to characterize the ligand binding specificity of different PDZ protein domains. The reported method "inverted peptide arrays", developed by us in 2004, is a prerequisit for that study. For the first time PDZ specificity could be determined in a proteomic context. The domains will be provided by our cooperation partners such as Prof Moelling, Dr. Linda Ball, Prof. Maden, or Prof. Fürst.
Head of Project:

Dr. rer. nat. Rudolf Volkmer
Charité - Universitätsmedizin Berlin
Institute of Medical Immunology CCM
Tel. 450-524267
Fax 450-524942
Additional Member of Project:

Dr. Prisca Boisguerin
Begin/End of Project:

08/2005 - 05/2009
Funded by:

Deutsche Forschungsgemeinschaft e.V.

Boisguerin P, Leben R, Ay B, Radziwill G, Moelling K, Dong L, Volkmer-Engert R. (2004) An improved method for the synthesis of cellulose membrane-bound peptides with free C termini is useful for PDZ domain binding studies. Chem Biol 11(4):449-459. Wiedemann U, Boisguerin P, Leben R, Leitner D, Krause G, Moelling K, Volkmer-Engert R, Oschkinat H. (2004) Quantification of PDZ domain specificity, prediction of ligand affinity and rational design of super-binding peptides. J Mol Biol 343(3):703-718