Identication of blood pressure regulating genes


Essential hypertension is a multifactorial disturbance of blood pressure regulation. Many environmental and genetic factors have been identified sofar. However the genetic basis of hypertension is not known. Blood pressure regulation due to genetic misregulation has not been demonstrated. The aim of the present study is to identify possible new bloodpressure regulating genes and their products using microarry-technology. We use a rat model with acute hypotension and wie induce regulation of bloodpressure increasing genes, which can be detected by microarray analysis. Differential regulated genes will be analyzed using Real-time PCR, RNA-in situ Hybridisation and Westernblotting. Identified gene will be functionally investigated. The identification of new blooo pressure regulating genes will possibly give new pharamcological possibilities to control blood pressure. In addition it will help to unterstand hypertension in more depth.
Keywords

Hypertonie; Genetik
Head of Project:

Dr. Timm Westhoff
Charité - Universitätsmedizin Berlin
Department of Endocrinology and Nephrology CBF
Nephrology
Additional Head of Project:

Prof. Dr. med. M. van der Giet
Begin/End of Project:

01/2005- 12/2005
Funded by:

Universitäre Forschungsförderung Charité
Publications:

Dietz R, Schomig A, Haebara H, et al. Studies on the pathogenesis of spontaneous hypertension in rats. Circ Res. 1978;43:I98-I106. 2. Suzuki H, Saruta T. An overview of blood pressure regulation associated with the kidney. Contrib Nephrol. 2004;143:1-15. 3. Caulfield M, Munroe P, Pembroke J, et al. Genome-wide mapping of human loci for essential hypertension. Lancet. Jun 21 2003;361(9375):2118-2123.