Expression of angio- and lymphangiogenetic growth factors on cells of solid pediatric tumors


Solid malignant tumors express angio- and lymphangiogenetic growth factors to promote their own blood supply and to induce lymphatic tumor dissemination. Recently the factors of the vascular endothelial growth factor family VEGF-A, -B, -C, -D and their receptors VEGFR-1, -2, -3 have been identified. The antagonization of these factors would lead to a new innovative therapy. For tumors of the pediatric age group, however, almost no data exist so far. Therefore, we want to analyse cells of established cell lines and of surgical samples in children who undergo surgery in our clinic because of neuro- or nephroblastoma or rhabdomyosarcoma for growth factor and receptor expression. We look for a possible correlation with tumor stage and prognosis of the children. An established, highly sensitive and quantitative RT-PCR is provided by a cooperation with the Medical Clinic (PD Höcker).
Head of Project:

PD.Dr. Winfried Barthlen
Charité - Universitätsmedizin Berlin
Department of Pediatric Surgery CVK
Tel. 030 / 450 566 553
Fax 030 / 450 566 905
winfried.barthlen@charite.de
Begin/End of Project:

01/2003- 12/2003
Funded by:

Universitäre Forschungsförderung Charité
Publications:

1. Plate, KH: From angiogenesis to lymphangiogenesis. Nature Med. 7:151-152, 2001 2. Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ, Holash J: Vascular-specific growth factors and blood vessel formation. Nature 407:242-248.2000 3. Ribatti D, Vacca A, Nico B, De Falco G, Montaldo PG, Ponzoni M: Angiogenesis and anti-angiogenesis in neuroblastoma. Eur J Cancer 38:750-757, 2002