Quantification of intraoperative tumor-cell spillage of neuroblastoma and rhabdomyosarkoma by FACS and RT-PCR


In pediatric patients with advanced solid tumors the minimal residual disease i.e. the persistence of vital tumor cells in the body after medical and surgical therapy is of utmost importance for the prognosis. It is conceivable that the inevitable manipulations of the tumor during the resection by the surgeon results in a tumor-cell spillage in the adjacent tissue and blood and lymphatic vessels. While this is an accepted fact for tumors of the adult population, there is no single study investigating this issue for tumors in childhood. In this clinical study we look for disseminated tumor cells in tissue fluid and peripheral blood before, during and after surgery for advanced neuroblastoma stage IV and mestastasizing alveolar rhabdomyosarcoma. Highly sensitive analytical methods like FACS and RT-PCR are applied which are provided by a cooperation with the Pediatric Clinic ( Dr. Lode). The proof of tumor cell spillage during the operation would be of great significance for surgical technique and perioperative medical treatment.
Head of Project:

PD.Dr. Winfried Barthlen
Charité - Universitätsmedizin Berlin
Department of Pediatric Surgery CVK
Tel. 030 / 450 566 553
Fax 030 / 450 566 905
winfried.barthlen@charite.de
Begin/End of Project:

01/2003- 12/2003
Funded by:

Universitäre Forschungsförderung Charité
Publications:

1. Burchill SA, Lewis IJ, Abrams KR, Riley R, Imeson J, Pearson AD, Pinkerton R, Selby P: Circulating neuroblastoma cells detected by reverse transcriptase polymerase chain reaction for tyrosine hydroxylase mRNA are an independent poor prognostic indicator in stage 4 neuroblastoma in children over 1 year. J Clin Oncol. 2001 Mar 15;19(6):1795-801 2. Smith LM, Anderson JR, Qualman SJ, Crist WM, Paidas CN, Teot LA, Pappo AS, Link MP, Grier HE, Wiener ES, Breneman JC, Raney RB, Maurer HM, Donaldson SS: Which patients with microscopic disease and rhabdomyosarcoma experience relapse after therapy ? A report from the soft tissue sarcoma committee of the children's oncology group. J Clin Oncol. 2001 Oct 15;19(20):4058-64. 3. Oliver RT: Does surgery disseminate or accelerate cancer ? Lancet. 1995 Dec 9;346(8989):1506-7 4. Douard R, Wind P, Sales JP, Cugnenc PH, Loric S: Malignant colorectal cell spread during no-touch cancer surgery.Surgery. 2000 Feb;127(2):237-8