Non-viral gene transfer of growth factor genes in ocular cells


Growth factors as intercellular messangers play a key role in wound healing. So far numerous growth factors have been characterized and their molecular structure has been clarified. The biological effects of some of them take place intracellularly. The availability of growth factors within the cell displays a main problem for specific manipulation of their activity. To target the cell membrane and the intracellular space liposomes have been shown to be very effective. In this project growth factor genes will be transfected into ocular cells, which possess only a limited capacity for proliferation under physiological conditions (corneal endothelium). Modulating the proliferation behavior of these cells via non-viral gene transfer could be helpful in developing new therapeutic approaches with clinical relevance for maintanence of vision.
Head of Project:

Haike Dannowski
Charité - Universitätsmedizin Berlin
Department of Ophthalmology CVK
Tel. 450-554035
Fax 450-554903
heike.dannowski@charite.de
Additional Head of Project:

Prof. Dr. Uwe Pleyer
Additional Member of Project:

Dr. P. Rieck
Begin/End of Project:

01/2003- 12/2003
Funded by:

Universitäre Forschungsförderung Charité
Publications:

Bednarz J, Teifel M, Friedl P, Engelmann K. Immortalization of human corneal endothelial cells using electroporation protocol optimized for human corneal endothelial and human retinal pigment epithelial cells. Acta Ophthalmol Scand. 2000, 78:130-6. Pleyer U, Groth D, Hinz B, Keil O, Bertelmann E, Rieck P, Reszka R. Efficiency and toxicity of liposome-mediated gene transfer to corneal endothelial cells. Exp Eye Res. 2001, 73:1-7. Schilling-Schon A, Pleyer U, Hartmann C, Rieck PW. The role of endogenous growth factors to support corneal endothelial migration after wounding in vitro. Exp Eye Res. 2000, 71:583-9.