Genetic characterization of rhabdomyosarcoma is there a difference concerning age group and tumor dissemination ?


Rhabdomyosarkoma is a soft tissue tumor of the adult and pediatric age group. It is characterized by a high local aggressivity and early dissemination. New innovative therapies can only be expected by molecular analysis of tumor cells, because conventional medical and surgical therapy has reached its margins. In this study surgical tumor samples are cut by microdissection and the gene expression is analyzed by microarray chip technique. Of special interest is the comparison of the gene expression of patients with early tumor dissemination with those who never develop distant disease. Furthermore we compare the gene expression of tumors of the adult with the pediatric group. The technique of microdissection and adult tumor tissue is provided by a cooperation with the Rober-Rössle-Clinic Berlin Buch (Prof. Schlag).
Keywords

Rhabdomyosarkom, Genanalyse
Head of Project:

PD.Dr. Winfried Barthlen
Charité - Universitätsmedizin Berlin
Department of Pediatric Surgery CVK
Tel. 030 / 450 566 553
Fax 030 / 450 566 905
winfried.barthlen@charite.de
Begin/End of Project:

01/2003- 12/2003
Funded by:

Universitäre Forschungsförderung Charité
Publications:

1. Scholl FA, Betts DR, Niggli FK, Schäfer BW: Molecular features of a human rhabdomyosarkoma cell line with spontaneous metastatic progression. Brit J Cancer 82:1239-1245, 2000 2. Yong-Jie Lu, Williamson D, Clark J, Wang R, Tiffin N, Skelton L, Gordon T, Williams R, Allen B, Jackman A, Cooper C, Pritchard-Jones K, Shipley J: Comparative expressed sequence hybridization to chromosomes for tumor classification and identification of genomic regions of differential gene expression. PNAS 98:9197-9202, 2001