Effects of argon on noise induced inner ear damage


Exposure to noise is known to cause cochlear damage with the consequence of hearing loss and tinnitus. The noise-induced hearing loss frequently includes frequency specific damage of the inner and outer hair cells (IHC, OHC). These cells do not regenerate, they are replaced by scar tissue. In previous in vitro experiments we observed a protective effect of Argon on hypoxia induced hair cell loss (Patent DE 10230616). Because hypoxia/ischemia plays an important pathogenetic role in the development of hearing loss it is possible that Argon acts protective on noise induced hearing loss. The present study is designed to investigate the effect of Argon on the damage of the inner ear in rats. The animals will be unilaterally exposed to an impulse noise series in a closed system, the contra lateral ear will be used as control. The evaluation of the damage occurs via Auditory brainstem responses (ABR) at frequencies of 0.5- 32 kHz and Distorsion product otoacoustic emissions (DPOAE) at frequencies of f2 = 1.5- 16 kHz. In addition a cochleogramm of the expression of mRNA of prestin and stereocilin will be determined. Positive results of the study will open up new preventive and therapeutic approaches for inner ear diseases.
Keywords

Lärm, Schwerhörigkeit, Tinnitus, Argon
Head of Project:

Prof.Dr.sc.med. Johann Gross
Charité - Universitätsmedizin Berlin
ENT Department CCM
Tel. 450-555311
Fax 450-555908
johann.gross@charite.de
Additional Member of Project:

Frau Haupt, Frau Dr. Mazurek, Dr. Iarine (Dresden)
Begin/End of Project:

01/2003- 12/2003
Funded by:

Universitäre Forschungsförderung Charité
Publications:

H.Haupt, F.Scheibe. Preventive magnesium supplement protects the inner ear against noise-induced impairment of blood flow and oxygenation in the guinea pig. Magnes.Res. 15 (2002) 17-25. H.Haupt, F.Scheibe, C.Ludwig, D.Petzold. Measurements of perilymphatic oxygen tension in guinea pigs exposed to loud sound. Eur.Arch.Otorhinolaryngol. 248 (1991) 413-416. J.Iarine, J.Gross, B.Mazurek: Verwendung von Edelgasen, insbesondere Argon, zur Prophylaxe oder Therapie von Innenohrerkrankungen. Patentschrift DE 10230616, 27.8. 2002